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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/08/2022 |
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Data da última atualização: |
25/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GIRARDI, E. A.; SOLA, J. G. P.; SCAPIN, M. da S.; MOREIRA, A. S.; BASSANEZI, R. B.; AYRES, A. J.; PEÑA, L. |
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Afiliação: |
EDUARDO AUGUSTO GIRARDI, CNPMF; JOÃO GABRIEL PANEGOSSI SOLA, FUND FOR CITRUS PROTECTION; MARCELO DA SILVA SCAPIN, FUND FOR CITRUS PROTECTION; ALECIO SOUZA MOREIRA, CNPMF; RENATO BEOZZO BASSANEZI, FUND FOR CITRUS PROTECTION; ANTONIO JULIANO AYRES, FUND FOR CITRUS PROTECTION; LEANDRO PEÑA, FUND FOR CITRUS PROTECTION. |
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Título: |
The perfect match: adjusting high tree density to rootstock vigor for improving cropping and land use efficiency of sweet orange. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Agronomy, v. 11, 2569, 2021. |
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ISSN: |
2073-4395 |
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DOI: |
https://doi.org/10.3390/agronomy11122569 |
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Idioma: |
Inglês |
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Conteúdo: |
The rise in the productivity of sweet orange in Brazil has been related to the use of superior rootstocks and higher tree density, among other factors. In order to investigate whether the cropping system and the land use efficiency would benefit from more intensive cultivation, the performance of Valencia sweet orange was evaluated over nine years on four rootstocks, which induced contrasting vigor, at 513, 696 and 1000 trees·ha?1. Agronomic Institute of Campinas (IAC) 1697 and IAC 1710 citrandarins, and diploid and allotetraploid (4×) Swingle citrumelos were classified as semi-dwarfing, super-standard, standard, and dwarfing rootstocks, respectively. The fruit yield per tree was decreased at higher tree densities, notably for more vigorous rootstocks. Conversely, the cumulative productivity was increased over the evaluation period by 27% at 1000 trees·ha?1, irrespective of the rootstock, and the most vigorous rootstock resulted in 2.5 times higher production than the dwarfing one on average. Most fruit quality parameters were seldom influenced by the tree density, while the rootstock was a decisive factor in improving the quality and the soluble solids content. Dwarfing rootstocks allowed for harvesting 17% more fruit per minute by manual pickers. Because the tree row volume per area is lower with such rootstocks, even at higher tree density, spray volume can be reduced, although appropriate equipment should be developed for better spray coverage on smaller trees. Nine years after planting under strict vector control, the cumulative incidence of huanglongbing-symptomatic trees on IAC 1710 was double that on Swingle 4×. Taken together, the results suggested that the land use efficiency in the citrus industry can be further improved by planting vigorous rootstocks at moderate to high tree densities. Nevertheless, obtaining highly productive semi-dwarfing and dwarfing rootstocks is the sine qua non for making high-density pedestrian sweet orange orchards more profitable. View Full-Text MenosThe rise in the productivity of sweet orange in Brazil has been related to the use of superior rootstocks and higher tree density, among other factors. In order to investigate whether the cropping system and the land use efficiency would benefit from more intensive cultivation, the performance of Valencia sweet orange was evaluated over nine years on four rootstocks, which induced contrasting vigor, at 513, 696 and 1000 trees·ha?1. Agronomic Institute of Campinas (IAC) 1697 and IAC 1710 citrandarins, and diploid and allotetraploid (4×) Swingle citrumelos were classified as semi-dwarfing, super-standard, standard, and dwarfing rootstocks, respectively. The fruit yield per tree was decreased at higher tree densities, notably for more vigorous rootstocks. Conversely, the cumulative productivity was increased over the evaluation period by 27% at 1000 trees·ha?1, irrespective of the rootstock, and the most vigorous rootstock resulted in 2.5 times higher production than the dwarfing one on average. Most fruit quality parameters were seldom influenced by the tree density, while the rootstock was a decisive factor in improving the quality and the soluble solids content. Dwarfing rootstocks allowed for harvesting 17% more fruit per minute by manual pickers. Because the tree row volume per area is lower with such rootstocks, even at higher tree density, spray volume can be reduced, although appropriate equipment should be developed for better spray coverage on smaller trees. Nine year... Mostrar Tudo |
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Thesagro: |
Laranja Doce; Planta Porta-Enxerto. |
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Thesaurus Nal: |
Oranges; Rootstocks. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02815naa a2200265 a 4500
001 2145759
005 2022-08-25
008 2021 bl uuuu u00u1 u #d
022 $a2073-4395
024 7 $ahttps://doi.org/10.3390/agronomy11122569$2DOI
100 1 $aGIRARDI, E. A.
245 $aThe perfect match$badjusting high tree density to rootstock vigor for improving cropping and land use efficiency of sweet orange.$h[electronic resource]
260 $c2021
520 $aThe rise in the productivity of sweet orange in Brazil has been related to the use of superior rootstocks and higher tree density, among other factors. In order to investigate whether the cropping system and the land use efficiency would benefit from more intensive cultivation, the performance of Valencia sweet orange was evaluated over nine years on four rootstocks, which induced contrasting vigor, at 513, 696 and 1000 trees·ha?1. Agronomic Institute of Campinas (IAC) 1697 and IAC 1710 citrandarins, and diploid and allotetraploid (4×) Swingle citrumelos were classified as semi-dwarfing, super-standard, standard, and dwarfing rootstocks, respectively. The fruit yield per tree was decreased at higher tree densities, notably for more vigorous rootstocks. Conversely, the cumulative productivity was increased over the evaluation period by 27% at 1000 trees·ha?1, irrespective of the rootstock, and the most vigorous rootstock resulted in 2.5 times higher production than the dwarfing one on average. Most fruit quality parameters were seldom influenced by the tree density, while the rootstock was a decisive factor in improving the quality and the soluble solids content. Dwarfing rootstocks allowed for harvesting 17% more fruit per minute by manual pickers. Because the tree row volume per area is lower with such rootstocks, even at higher tree density, spray volume can be reduced, although appropriate equipment should be developed for better spray coverage on smaller trees. Nine years after planting under strict vector control, the cumulative incidence of huanglongbing-symptomatic trees on IAC 1710 was double that on Swingle 4×. Taken together, the results suggested that the land use efficiency in the citrus industry can be further improved by planting vigorous rootstocks at moderate to high tree densities. Nevertheless, obtaining highly productive semi-dwarfing and dwarfing rootstocks is the sine qua non for making high-density pedestrian sweet orange orchards more profitable. View Full-Text
650 $aOranges
650 $aRootstocks
650 $aLaranja Doce
650 $aPlanta Porta-Enxerto
700 1 $aSOLA, J. G. P.
700 1 $aSCAPIN, M. da S.
700 1 $aMOREIRA, A. S.
700 1 $aBASSANEZI, R. B.
700 1 $aAYRES, A. J.
700 1 $aPEÑA, L.
773 $tAgronomy$gv. 11, 2569, 2021.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
10/01/2012 |
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Data da última atualização: |
22/04/2021 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LOBO, I. K. C.; HANADA, R. E.; SOUSA, N. R.; GASPAROTTO, L.; SOUZA Jr. M. T.; KEMA, G. H. J.; SILVA, G. F. da. |
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Afiliação: |
I. K. C. Lobo; R. E. Hanada, INPA; NELCIMAR REIS SOUSA, CPAA; LUADIR GASPAROTTO, CPAA; EMBRAPA AGROENERGIA; GILVAN FERREIRA DA SILVA, CPAA. |
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Título: |
Construction of gateway binary vector for selection with bialaphos or carboxin and GFP expression in fungi. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE GENÉTICA, 57., 2011, Águas de Lindóia. Resumos... s.l.: SBG, 2011. p. 25. |
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Idioma: |
Português |
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Conteúdo: |
Genomic data has created a growing demand for tools and methodologies for studying the genes function, which can be realized through loss of function experiments (gene knockout) or by RNA silencing (knockdown). The develop-ment of binary vectors for Agrobacterium tumefaciens mediated transformation (ATMT) has the advantage of being independent of protoplast formation and can be used directly on a wide variety of fungal species and tissue types. The selection of transformants using bialaphos and carboxin has the advantages of low cost in the transformation and availability of different selectable markers, also allowing the analysis of several genes and combination of study by knockout or knockdown, using selectable markers in the same transformant. Thus, this study aimed to build two binary vectors containing reporter gene and selectable markers that confer resistance to carboxin and bialaphos. The cassettes were constructed using the Gateway system to two fragments. The gene encoding the GFP protein and PtoxA and PtrpCpromoters were cloned into pDONR P1-P5R plasmid. Genes that confer bialaphos and carboxin resistance, bar and cbxr respectively, were cloned into pDONR P5-P2 plasmid. The pPGW plasmid was used as des-tination vector. The gfp gene transcription is controlled by PtoxA promoter and the bar and cbxr genes transcriptions are controlled by PtrpC promoter. These binary vectors were named pGWGFP-BAR and pGWGFP-CBXR. The assembly of cassettes was confi rmed by sequencing, and the validation of vectors is being accomplished through transformation (ATMT) with the plant pathogens Mycosphaerella fi jiensis and Fusarium oxysporum f. sp. cubense. MenosGenomic data has created a growing demand for tools and methodologies for studying the genes function, which can be realized through loss of function experiments (gene knockout) or by RNA silencing (knockdown). The develop-ment of binary vectors for Agrobacterium tumefaciens mediated transformation (ATMT) has the advantage of being independent of protoplast formation and can be used directly on a wide variety of fungal species and tissue types. The selection of transformants using bialaphos and carboxin has the advantages of low cost in the transformation and availability of different selectable markers, also allowing the analysis of several genes and combination of study by knockout or knockdown, using selectable markers in the same transformant. Thus, this study aimed to build two binary vectors containing reporter gene and selectable markers that confer resistance to carboxin and bialaphos. The cassettes were constructed using the Gateway system to two fragments. The gene encoding the GFP protein and PtoxA and PtrpCpromoters were cloned into pDONR P1-P5R plasmid. Genes that confer bialaphos and carboxin resistance, bar and cbxr respectively, were cloned into pDONR P5-P2 plasmid. The pPGW plasmid was used as des-tination vector. The gfp gene transcription is controlled by PtoxA promoter and the bar and cbxr genes transcriptions are controlled by PtrpC promoter. These binary vectors were named pGWGFP-BAR and pGWGFP-CBXR. The assembly of cassettes was confi rmed by sequencin... Mostrar Tudo |
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Thesagro: |
Genética. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/51989/1/Resumo-Igor-co-autor.pdf
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Marc: |
LEADER 02305nam a2200193 a 4500
001 1912252
005 2021-04-22
008 2011 bl uuuu u00u1 u #d
100 1 $aLOBO, I. K. C.
245 $aConstruction of gateway binary vector for selection with bialaphos or carboxin and GFP expression in fungi.
260 $aIn: CONGRESSO BRASILEIRO DE GENÉTICA, 57., 2011, Águas de Lindóia. Resumos... s.l.: SBG, 2011. p. 25.$c2011
520 $aGenomic data has created a growing demand for tools and methodologies for studying the genes function, which can be realized through loss of function experiments (gene knockout) or by RNA silencing (knockdown). The develop-ment of binary vectors for Agrobacterium tumefaciens mediated transformation (ATMT) has the advantage of being independent of protoplast formation and can be used directly on a wide variety of fungal species and tissue types. The selection of transformants using bialaphos and carboxin has the advantages of low cost in the transformation and availability of different selectable markers, also allowing the analysis of several genes and combination of study by knockout or knockdown, using selectable markers in the same transformant. Thus, this study aimed to build two binary vectors containing reporter gene and selectable markers that confer resistance to carboxin and bialaphos. The cassettes were constructed using the Gateway system to two fragments. The gene encoding the GFP protein and PtoxA and PtrpCpromoters were cloned into pDONR P1-P5R plasmid. Genes that confer bialaphos and carboxin resistance, bar and cbxr respectively, were cloned into pDONR P5-P2 plasmid. The pPGW plasmid was used as des-tination vector. The gfp gene transcription is controlled by PtoxA promoter and the bar and cbxr genes transcriptions are controlled by PtrpC promoter. These binary vectors were named pGWGFP-BAR and pGWGFP-CBXR. The assembly of cassettes was confi rmed by sequencing, and the validation of vectors is being accomplished through transformation (ATMT) with the plant pathogens Mycosphaerella fi jiensis and Fusarium oxysporum f. sp. cubense.
650 $aGenética
700 1 $aHANADA, R. E.
700 1 $aSOUSA, N. R.
700 1 $aGASPAROTTO, L.
700 1 $aSOUZA Jr. M. T.
700 1 $aKEMA, G. H. J.
700 1 $aSILVA, G. F. da
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Embrapa Amazônia Ocidental (CPAA) |
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